Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: 99m Tc-(HE) 3 -(GPO) 9 SPECT/CT in MI. (A) Illustrative examples of in vivo SPECT/Exitron Nano-12000–enhanced CT images of sham-operated and MI-induced mice at 2 wk after surgery. Arrows in middle column indicate 99m Tc-(HE) 3 -(GPO) 9 uptake in infarct zone of MI-induced mice, identified by 99m Tc-tetrofosmin MPI (top row). Single asterisks mark 99m Tc-(HE) 3 -(GPO) 9 uptake at surgical site in both sham and MI-induced mice. (B) Quantification of 99m Tc-(HE) 3 -(GPO) 9 SPECT signal in infarct and remote zones of MI-induced mice and corresponding walls of sham-operated mice. ## P < 0.01 (Mann–Whitney U test); #### P < 0.0001 (Mann–Whitney U test); **** P < 0.0001 (Wilcoxon signed-rank test).

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: Single Photon Emission Computed Tomography, In Vivo, MANN-WHITNEY

Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: 99m Tc-(HE) 3 -(GPO) 9 autoradiography in MI. (A) Illustrative examples of Masson trichrome staining, Sirius red staining, and 99m Tc-(HE) 3 -(GPO) 9 autoradiography (after in vivo tracer administration) of sham-operated and MI-induced mice 2 wk after surgery. (B) Quantification of 99m Tc-(HE) 3 -(GPO) 9 uptake by autoradiography in infarct and remote zones of MI-induced mice and corresponding walls of sham-operated mice. (C) Correlation between 99m Tc-(HE) 3 -(GPO) 9 signals on SPECT/CT and autoradiography in infarct zone and anterolateral wall of sham-operated mice. # P < 0.05 (Mann–Whitney U test); * P < 0.05 (Wilcoxon signed-rank test); ## P < 0.01 (Mann–Whitney U test).

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: Autoradiography, Staining, In Vivo, Single Photon Emission Computed Tomography, MANN-WHITNEY

Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: 99m Tc-(HE) 3 -(GPO) 9 specificity. (A) Illustrative examples of in vivo ceCT using eXIA 160XL fused with Exitron Nano-12000–enhanced CT (top row), along with SPECT/Exitron Nano-12000–enhanced CT of 99m Tc-(HE) 3 -(GPO) 9 (middle row) and 99m Tc-(HE) 3 -(GPO) SCR (bottom row) in MI-induced mice at 2 wk after surgery. Arrows point to scarred area. (B) Quantification of 99m Tc-(HE) 3 -(GPO) 9 SPECT signal in infarct zone of MI-induced mice compared with 99m Tc-(HE) 3 -(GPO) 9 signal in remote zone (top graph) and 99m Tc-(HE) 3 -(GPO) SCR signal in infarct zone (bottom graph) of same mice. (C) Illustrative examples of Masson trichrome staining, Sirius red staining, and 99m Tc-(HE) 3 -(GPO) SCR autoradiography (after in vivo tracer administration) of MI-induced mice 2 wk after surgery. (D) Quantification of 99m Tc-(HE) 3 -(GPO) SCR uptake by autoradiography in infarct zone of MI-induced mice compared with 9m Tc-(HE) 3 -(GPO) SCR uptake in remote zone (top graph) and 99m Tc-(HE) 3 -(GPO) 9 uptake in infarct zone (bottom graph) of same animals. * P < 0.05 (Wilcoxon signed-rank test); ## P < 0.01 (Mann–Whitney U test).

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: In Vivo, Single Photon Emission Computed Tomography, Staining, Autoradiography, MANN-WHITNEY

Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: (A) Illustrative examples of Sirius red staining, 99m Tc-(HE) 3 -(GPO) 9 autoradiography (after in vivo tracer administration), and denatured collagen staining of sham-operated and MI-induced mice at 2 wk after surgery using R-CHP. (B) Quantification of R-CHP staining in MI-induced and sham-operated animals. (C) Correlation between 99m Tc-(HE) 3 -(GPO) 9 uptake by SPECT/CT (top) or autoradiography (bottom), and R-CHP staining intensity in infarct zone. RFU: relative fluorescence unit. * P < 0.05 (Wilcoxon signed-rank test); ### P < 0.001 (Mann–Whitney U test).

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: Staining, Autoradiography, In Vivo, Single Photon Emission Computed Tomography, Fluorescence, MANN-WHITNEY

Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: Fibrosis-related imaging targets over time. Illustrative examples (A) and infarct–to–remote area signal intensity ratios (B) of ex vivo 99m Tc-(HE) 3 -(GPO) 9 autoradiography, R-CHP staining, procollagen staining, and ex vivo 99m Tc-RYM1 autoradiography in adjacent sections of hearts collected from controls and from MI-induced mice at 3 d, 1 wk, and 2 wk after MI. Sirius red images are shown as reference in panel A. AD = applied dose. # P < 0.05 (Kruskal–Wallis test); ## P < 0.01 (Kruskal–Wallis test).

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: Imaging, Ex Vivo, Autoradiography, Staining

Journal: Journal of Nuclear Medicine

Article Title: Molecular Imaging of Collagen Turnover in Myocardial Infarction

doi: 10.2967/jnumed.125.271721

Figure Lengend Snippet: 99m Tc-(HE) 3 -(GPO) 9 binding to human myocardial tissue. Illustrative examples of Masson trichrome staining of fibrosis, Sirius red staining of collagen, denatured collagen staining using R-CHP, and 99m Tc-(HE) 3 -(GPO) 9 autoradiography in normal and fibrotic myocardium. DAPI = 4′,6-diamidino-2-phenylindole.

Article Snippet: To address tracer uptake specificity, a subset of animals underwent 99m Tc-(HE) 3 -(GPO) 9 SPECT (23.3 ± 6.7 MBq) imaging, followed by 99m Tc-(HE) 3 -(GPO) SCR SPECT (26.3 ± 6.3 MBq) within 2 or 3 d. Each SPECT study was coupled with either eXIA 160XL enhanced CT or Exitron Nano-12000 enhanced CT. MILabs software version 12 was used to reconstruct contrast-enhanced CT (ceCT) images at an isotropic voxel size of 0.1 mm using a filtered backprojection algorithm.

Techniques: Binding Assay, Staining, Autoradiography

Journal: Journal of Nuclear Medicine

Article Title: Development of an Optimized CXCR4-Targeting Theranostic Pair

doi: 10.2967/jnumed.125.269933

Figure Lengend Snippet: (A) Maximum-intensity projection SPECT/CT images of Z138-bearing NRG male mouse injected with [ 177 Lu]Lu-BL34 and imaged longitudinally at 1, 4, 24, and 72 h after injection. (B) Ex vivo biodistribution studies at 1, 4, 24, and 72 h after injection of [ 177 Lu]Lu-BL34 in Z138-bearing NRG male mice. p.i. = postinjection.

Article Snippet: PET/CT scans were performed on a Siemens Inveon, whereas SPECT/CT images were obtained using a MILabs scanner.

Techniques: Single Photon Emission Computed Tomography, Injection, Ex Vivo

Journal: Life

Article Title: Distinct Biodistribution of Natural Killer Cell-Derived Exosomes in an Orthotopic A549 Lung Cancer Mouse Model: Implications for Potent Targeted Drug Delivery

doi: 10.3390/life16040654

Figure Lengend Snippet: In vitro validation of DiR dye labeling efficiency on Exos. Representative fluorescence images of DiR-labeled Exos captured using the MILabs optical imaging system. The experiment included control groups (PBS only, and unlabeled Exo only) and experimental groups with increasing concentrations of Exos (2 × 10 10 , 6 × 10 10 , and 1 × 10 11 particles) labeled with a fixed amount of DiR dye. The red box highlights the optimal labeling condition (2 × 10 10 Exo + 5 µL DiR) used for the follow-up animal experiments.

Article Snippet: Six hours (15 min, 1 h, 2 h, 4 h, 6 h) post-intravenous injection, ex vivo biodistribution was assessed using the MILabs Spectrum imaging system.

Techniques: In Vitro, Biomarker Discovery, Labeling, Fluorescence, Optical Imaging, Control